HPV Infection and HPV Subtypes in Normal and Abnormal Cervical Cytology in Turkish Women

Objective: To investigate the prevalence of HPV infection and the distribution of HPV subtypes, as well as cervical dysplasias in Turkey. Study Design: Through the years 2006-2008, 2161 women who have admitted to gynecology clinics were evaluated for cervical cytology, demographical characteristics and HPV infection. Results: Considering all the cases, 94,4 % (n=2040) had normal cytology, 3,8 % (n=82) had LSIL and 1,8 % (n=39) had HSIL. In the cytological evaluation, 8,75% (n=189; n=115 HPV(+), n=74 HPV(-)) of the patients were diagnosed as ASCUS. The incidence of HPV positivity was 12,6% (n=272). The most common subtype was HPV type 6 (n=109, 5%) while the incidences of the other subtypes from second most common to least common were as follows; type 18 (n=63, 2,9%), type 16 (n=53, 2,4%), type 11 (n=20, 0,9%), type 45 (n=10, 0,4%), type 52 (n=10, 0,4%), type 59 (n=4, 0,18%) and type 51 (n=3, 0,14%). Among the HPV positive patients, the most common HPV subtype was type 6 (n=109, 40%), and the other subtypes were type 18 (n=63, 23,2%), type 16 (n=53, 19,5%), type 11 (n=20, 7,3%), type 45, 51, 52 and 59. Conclusions: Eighty-nine percent of the HSIL smears were positive for HPV subtype 16 and/or 18. This suggests that by the use of vaccination programs, a significant amount of precancerous cervical lesions and cancer might be prevented.


Introduction
Cervical cancer, major cancer affecting women in developing countries, is caused by the persistent infection of "high-risk" genotypes of human papilloma viruses (HPV).Human papilloma viruses are DNA viruses that infect basal epithelial (skin or mucosal) cells.HPV is highly transmissible, with the peak incidence after the onset of sexual activity.Clinical and subclinical HPV infections are the most common sexually transmitted diseases today.There are more than 100 known HPV genotypes, at least 15of which can cause cancer of either cervix or other sites.There is an international consensus indicating that "high-risk" genotypes, including genotypes 16,18,31,33,35,39,45,51,52,56,58,59 and 66, could lead to cervical cancer.Among all, 16 and 18, the two most common oncogenic types, cause approximately 70% of all cervical cancers, worldwide, while HPV 6 and 11, can also cause genital warts.On the other hand, as not all the cervical HPV infection is symptomatic, 5-40% of women at reproductive age might be infected by HPV without suffering any symptoms (1-4).There have been many studies worldwide on the proportion of cervical cancer, highand low-grade squamous intraepithelial lesions (HSIL and LSIL) due to different HPV genotypes (5-9).
HPV vaccine that is avaliable for clinical use, was able to be developed in bivalent and quadivalent forms.The vaccines are successful in the profilaxis of 70 % of all cervical cancers.By the use of quadrivalent vaccine about 90 % of genital wart formation can be prevented.In the clinical studies, HPV vaccine has been shown to close to 100 % protective against the formation of genital wart, precancerous and cancerous cervical lesions caused by HPV type 6,11,.

Materials and Methods
Through the years 2008-2010, 2161 women who have admitted to gynecology clinics in Ankara Bayındır private hospital, İstanbul Bilim University Hospital and İstanbul GATA military hospital were evaluated for cervical cytology, demographical characteristics and HPV infection.In order to assess the cervical cytology, the samples collected from cervical os and posterior vaginal fourcette by a brush, were fixed and send to the pathology laboratory.The women were conducted into subgroups, normal, ASCUS, HSIL, LSIL according to cervical cytology.The cervical samples of the patients who had genital warts and were suspected to have HPV infection (perinuclear halo and coilocytosis in cervical smear) by the evaluation of their cervical cytology, were collected for the HPV DNA scanning and HPV typing.It were made to HPV DNA and subtype analysis to patients who had suspected of HPV infection.HPV DNA analysis were not made to all patients.For this purpose, the samples were collected from cervical ostium by the help of a brush and send to the pathology laboratory in ''The Thin Prep Preserv Cyt solution 20 ml cytyc corp'' solution.
By using the primers specific for the E6 and E7 regions of the HPV subtypes 6,11,16,18,31,33,35,45,51,52,56,58,59, 68, multiplex fluoresans polymerase chain reaction (PCR) performed in order to screen HPV DNA and determine the type .One hundred and eighty nine cases who received ASCUS diagnosis by cervial cytological evaluation were undergone for reevaluation colposcopic observation and/or cervical biopsies.Thus, by the results of the reevaluation, the patients with ASCUS diagnosis have been moved into groups either normal, HSIL or LSIL.According to the HPV prescence, the normal, HSIL and LSIL groups were subdivided.Each patient was questioned for their number of delivery.All results were expressed as the mean ± StDev.Two independent groups were compared with Mann-Whitney U-test.A p value of less than 0.05 was considered statistically significant.All participants' rights were protected and informed consents were obtained according to the Helsinki Declaration.The study protocol was approved by local Ethic Committee.
Distribution of the HPV subtypes according to the age groups has been shown (Figure 2).
There was no significant correlation between HPV positivity and parity (p>0,05).

Discussion
Genital HPV infection is the most common sexually transmitted disease.Taking into consideration of all the cervical cancer cases, 99,8% of the patients were infected by HPV.HPV infection may cause either genital wart, precancerous cervical lesions or cervical cancer.While more than 100 HPV subtypes exist, 30 subtypes may lead to infection in human.According to the oncogenicity potential, HPV types are classified as high risk (HR; type 16,18,31,45,51,52,59,68) and low risk (LR; type 6 and 11) (1-4).
HPV infection prevalence exhibits regional variations.The incidence of HPV among 14-59 years of age Americans is 26,8% and the most common HPV subtype is type 6 (1,3%) (14), while this incidence is 17% in Spaniens and the most common HPV subtype is type 16 and 18 (15).On the other hand, among Indian women, type 16 is the most common HPV subtype and the incidence is 7,6% (16), in Denmark the percentage of the HPV infected women is 26,4 % type 16 is the most common one (6,0%) (17).In our study, screening 2161 patients, the incidence of HPV infection was found to be 12,6 % (n=272) and the most common HPV type was type 6 (n=109, 5%).
In our population group, although the HPV infection was most common in ages between 33 and 38 (n=58, 21,3%), no correlation has been found between the HPV infection incidence and the age (p>0,05).Among all the age groups, HPV type 6 was the most common.The highest frequency of HPV was in the age group 24-29 in USA (27,5%) 14 and 20-25 in Denmark (50,2 %) 17 .In our study, the HPV infection was more common in the primipar and multipar (the women who had two delivery) women groups compared to the others.
At present, bivalant (Cervarix-type 16, 18) and quadrivalant 11,16,18) vaccines are ready to use in the clinical practice and they were shown to be 100% effective in the profilaxis of the HPV subtypes in their spectrum.88,8% of the HSIL are caused by type 16 and 18.Thus, by the use of the appropriate vaccination programs, the significant amount of the precancerous cervical lesions and cervical cancer that might be prevented.