@article{dantas2014biofilm,
  title = {Biofilm Formation in Denture Base Acrylic Resins and Disinfection Method Using Microwave},
  author = {Ana Patricia Fernandes Macedo Dantas and Rafael Leonardo Xediek Consani and Janaina de Cassia Orlandi Sardi and Marcelo Ferraz Mesquita and Manoela Capla de Vasconcellos dos Santos da Silva and Mario Alexandre Coelho Sinhoreti},
  year = 2014,
  url = {https://ibimapublishing.com/articles/DENT/2014/112424/},
  journal = {Journal of Research and Practice in Dentistry},
  volume = 2014 (2014),
  pages = 9,
  doi = 10.5171/2014.112424,
  abstract = {The purpose of this study was to verify the effect of the microwave disinfection in denture base acrylic resins with Candida sp biofilm formation. Samples (n=90) of each resin were submitted to ethylene oxide sterilization, and then infected by inoculum of yeast (C. albicans, C. tropicalis and C. dubliniensis) with 200 Î¼L of cultures adjusted to 108 CFU/mL, placed in specific media and aerobically incubated at 37°C for 24 hours for biofilm formation. For each resin and microorganism, samples were irradiated by microwave energy at 650 W for 3 minutes. Non-irradiated samples and without microorganisms samples were considered as control. The samples were washed with saline solution and plated in Sabouraud dextrose agar (SDA) for analysis of the number of colony forming units (CFU/mL). Data were submitted to Kruskal-Wallis followed by Student-Newman-Keuls test (α=0.05). The results demonstrated a significant decrease in the number of viable colonies for all biofilms formed by Candida species. It was demonstrated an effective disinfection of the samples contaminated by Candida biofilm when microwave irradiated. This study demonstrated that microwave disinfection was able to significantly decrease the amount of microorganisms from denture base acrylic resins contaminated and therefore could be used as a preventive method of candidiasis caused by dentures.},
  keywords = {Acrylic resin, Candida sp, biofilm, disinfection, microwave.},
  note = Article ID: 112424
}
