@article{sato2013hela,
  title = {Hela Cells Consist of Two Cell Types, as Evidenced by Cytochemical Staining for Alkaline Phosphatase Activity: A Possible Model for Cancer Stem Cell Study},
  author = {Masahiro Sato and Naoko Kubota and Emi Inada and Issei Saitoh and Masato Ohtsuka and Shingo Nakamura and Takayuki Sakurai and Satoshi Watanabe},
  year = 2013,
  url = {https://ibimapublishing.com/articles/ASC/2013/208514/},
  journal = {Advances in Stem Cells},
  volume = 2013 (2013),
  pages = 13,
  doi = 10.5171/2013.208514,
  abstract = {Alkaline phosphatase (ALP) is known to be expressed in the several somatic stem cells and cancer cells. To investigate whether ALP may be a promising marker for cancer stem cells (CSCs), we examined the expression of ALP in human squamous cell carcinoma HeLa cells using a cytochemical staining kit. We found that approximately 40% of HeLa cells were positive for ALP activity. A single cell-derived colony assay revealed that the newly formed colonies could be classified into uniformly (U, 23%), mosaically (M, 17%), and non-stained (N, 60%) colonies. Each colony was picked and cultured for 2 additional weeks for cell propagation; the cells were either M- (45%) or N-type (55%), suggesting that the U-type colonies may have spontaneously changed to M-type colonies during cultivation. These resulting M- or N-type cells were stable with respect to ALP activity. DNA microarray analysis revealed that the gene expression pattern of N-type cells is almost identical to that of their parental HeLa cells (comprising M-type cells), but several genes including ALP gene were upregulated in the HeLa cells. Cultivation of single HeLa cell-derived colonies in the presence of the small molecule 6-bromoindirubin-3'-oxime (BIO), a potent inhibitor of glycogen synthase kinase 3 (GSK3), caused a reduction in the ratio of M-type colonies, suggesting that the transition from U- to M-type colonies is regulated by the Wnt/-catenin signaling pathway. Although there is no evidence, at present, that the ALP-positive cells are CSCs, future investigation may reveal that HeLa cells may be a good model for CSC study.},
  keywords = {Cancer stem cell; alkaline phosphatase; mosaic expression; HeLa cells.},
  note = Article ID: 208514
}