@article{ali2014differentiation,
  title = {Differentiation of Human Embryonic Stem Cells towards a Sensory Neural Phenotype},
  author = {Rouknuddin Qasim Ali and Yen-Fu Cheng and Beata Kostyszyn and Lars Ährlund-Richter and Mats Ulfendahl},
  year = 2014,
  url = {https://ibimapublishing.com/articles/ASC/2014/252491/},
  journal = {Advances in Stem Cells},
  volume = 2014 (2014),
  pages = 12,
  doi = 10.5171/2014.252491,
  abstract = {Sensorineural hearing loss, caused by the degeneration of inner ear sensory hair cells or afferent auditory neurons, is a major problem in clinical medicine. In birds and lower vertebrates, regeneration of these cells may occur but in mammals, the loss is irreversible. In this study, neuronal progenitors were derived from human embryonic stem cells in the form of neural tube-like rosettes and harvested enzymatically after 4 days (NS4), 7 days (NS7) or 11 days (NS11). This was followed by a further differentiation on laminin-coated plates for 1 or 3 weeks in neural induction medium without bFGF. Such cultures were all dominated by cells immunoreactive for nestin, a common neuronal progenitor marker, as well as for Tuj1, an early marker for neurons.
Among the combinations tested, the NS7 + 1 week protocol showed the most abundant induction of the sensory neuron markers TrkC (20%) and peripherin (11%), making this protocol the most favorable for the induction of sensory neurons. Compared with other published more complex protocol requiring the addition of different growth factors, the straightforward approach described here may contribute with a simplified starting point for the further exploration of novel approaches for in vitro generation of sensory neurons, and ultimately the treatment of sensory hearing impairment using cell transplantation.},
  keywords = {hESC, sensory neurons, in-vitro.},
  note = Article ID: 252491
}