@article{samoilova2014pcrbased,
  title = {PCR-based Identification of Erwinia amylovora Bacteriophages isolated in the Republic of Moldova},
  author = {Anna V. Samoilova and Andreas Leclerque},
  year = 2014,
  url = {https://ibimapublishing.com/articles/JVM/2014/293991/},
  journal = {Journal of Virology and Microbiology},
  volume = 2014 (2014),
  pages = 9,
  doi = 10.5171/2014.293991,
  abstract = {Fire blight of Rosaceae plants, one of the economically most important diseases of fruit trees, is caused by the bacterial pathogen Erwinia amylovora. Alternatives to the currently available, mostly unspecific fire blight control strategies are highly solicited. Bacteriophages from the virus families Podoviridae, Myoviridae, and Siphoviridae that infect and lyse E. amylovora bacteria, are under intensive evaluation as possible highly specific biocontrol agents. Myoviridae, and Siphoviridae (termed collectively M7 group phages), on the one hand, and Podoviridae (belonging to the L1 group of bacteriophages), on the other hand, differ considerably in their relevant properties as, e.g., host range and efficiency of infection. In this study, E. amylovora infecting viruses were isolated from Rosaceae fruit trees in the municipality of Chisinau, Republic of Moldova. Based on the comparison of published E. amylovora phage genome sequences, the viral terminase large subunit encoding tls gene was identified as a suitable marker for the genetic characterization of E. amylovora associated bacteriophages that allows distinguishing positively between L1 and M7 group phages. A PCR-based diagnosis protocol was established and employed with a set of 13 Moldovan phage isolates. Nine of these were demonstrated to belong to the M7 group of phages, whereas four isolates could not be assigned to either of both phage groups.},
  keywords = {Fire Blight; Erwinia amylovora bacteriophages; Biological Control; Genetic Characterization},
  note = Article ID: 293991
}