@article{braglia2015isolation,
  title = {Isolation of a Sequence Homolog to More Axillary Branches MAX2 Gene in Hibiscus rosa-sinensis and its Use as Genetic Marker},
  author = {Luca Braglia and Federica Nicoletti and Laura De Benedetti and Nicola Pecchioni and Antonio Mercuri},
  year = 2015,
  url = {https://ibimapublishing.com/articles/AGRI/2015/360263/},
  journal = {Research in Agriculture and Agronomy},
  volume = (2015),
  pages = 8,
  doi = DOI: 10.5171/2015.360263,
  abstract = {Lateral branching plays an important role in the elaboration of adult plants architecture. Herein, we adopted a modified AFLP approach combined with a degenerate primer amplification to identify and isolate in the underinvestigated ornamental species H. rosa-sinensis an orthologous element of the MAX2 gene (More Axillary Branches), which acts downstream of the branching inhibition signaling pathway. A specific gene fragment was cloned and sequenced from nineteen H. rosa-sinensis cultivars and twelve Hibiscus botanical species and different significant nucleotide polymorphisms among genotypes that were observed. The comparative analysis revealed a high conservation of DNA sequences among cultivars and wild species sexually compatible with H. rosa-sinensis. The deduced amino acid sequences of the Hibiscus isolated fragments reveal four characteristic repeat regions showing high identity with other F-box/Leucine Reach Repeat MAX2 homologous sequences. The cloned fragment is a likely candidate gene to be validated for association with phenotype to release a gene-derived "perfect marker" for the compact habit trait.},
  keywords = {Hibiscus - AFLP approach - ornamentals - shoot branching.},
  note = Article ID: 360263
}