@article{vanghele2016association,
  title = {Association of DnaK and GroEL with Antimicrobial Resistance in Salmonella Abortusovis},
  author = {Luminita Monica Vanghele and Maria Ionescu and Handan Coste and Elena Ganea},
  year = 2016,
  url = {https://ibimapublishing.com/articles/IJVMR/2016/623135/},
  journal = {International Journal of Veterinary Medicine: Research & Reports},
  volume = (2016),
  pages = 11,
  doi = 10.5171/2016.623135,
  abstract = {Multidrug-resistant (MDR) strains of Salmonella are now encountered frequently and the rates of multidrug-resistance have increased considerably in recent years. The association of DnaK and GroEL molecular chaperones with the stress response induced by antibiotics has been mentioned for the most frequently isolated serovar, S. enterica subsp. enterica Typhimurium, but similar studies on S. Abortusovis were not published, so far.  In the present work, the induction of DnaK and GroEL in S. Abortusovis cells under stress conditions caused by heat shock and by various antibiotics respectively was studied by Western blot and real time RT-PCR methods. The transcription and translation analyses showed increased levels of DnaK and GroEL after a temperature shift from 37°C to 42°C, and after the exposure of a multiresistant S. Abortusovis strain to subinhibitory concentrations of various antimicrobials (nalidixic acid, streptomycin, kanamycin, tetracycline and gentamicin). The data presented here may provide a starting point for future research regarding the role of S. Abortusovis molecular chaperones in the stress response induced by antibiotics.     },
  keywords = {Gene expression , Salmonella Abortusovis , molecular chaperones, antimicrobial resistance},
  note = Article ID: 623135
}