@article{ismail2014p53,
  title = {p53 Expression Immuno-histochemistry Index in Stage III Giant Cell Tumor of the Bone},
  author = {Faisham W. Ismail and Ng Thong Pin and M. Z. Nor Azman and Zulmi Wan and M. .D Salzihanand Mutum S. Samarendra},
  year = 2014,
  url = {https://ibimapublishing.com/articles/JMED/2014/675393/},
  journal = {JMED Research},
  volume = 2014 (2014),
  pages = 7,
  doi = 10.5171/2014.675393,
  abstract = {We analyzed the p53 expression of 50 consecutive cases of stage III giant cell tumor to determine the clinico-pathological correlation. There were 29 male patients compared to 21 females. The mean age was 36.42 years and ranged from 19 to 66 years. Tumors were located mainly around the knee (48%) followed by distal radius (28%). Sixteen percent (16%) had local recurrence, and twenty four percent (24%) presented with or eventually developed lung metastasis. There were 3 patients who had both local recurrence and pulmonary metastasis. The expression of p53 antigen was evaluated by immuno-histochemical staining techniques, using the avidin-biotin perioxidase complex method, using an LSAB2 kit (Dako, Glostrup, Denmark). The scoring was done by counting the positively stained cell in the background of 1000 mononuclear stromal cells. Data was analyzed using PASW version 18.0. The mean value of p53 index obtained as a percentage of 1000 background cells was 37.68 (ranged 1.00 — 20.0), with standard deviation of 28.33. The p53 index of recurrent tumor was 65.38 compared to 32.42 of those without recurrence, and was statistically significant (mean difference of 32.97 with p value in independent t test of 0.002). The p53 index was also statistically significant in the presence of pulmonary metastases with the mean value of 66.92 compared to 28.45 of those without metastasis (mean difference of 38.47 with p value in independent t test of 0.001). In conclusion, p53 expression is a good prognostic marker to predict the risk of local recurrence and lung metastasis in the giant cell tumor of the bone.},
  keywords = {p53, staged III, giant cell tumor of the bone.},
  note = Article ID: 675393
}