@article{ohalloran2014cellular,
  title = {Cellular Responses to Chondroitin-6-Sulphate Releasing Brushite Bone Cements},
  author = {Michael O'Halloran },
  year = 2014,
  url = {https://ibimapublishing.com/articles/DENT/2014/730677/},
  journal = {Journal of Research and Practice in Dentistry},
  volume = 2014 (2014),
  pages = 19,
  doi = 10.5171/2014.730677,
  abstract = {As the field of implant dentistry continually develops, clinicians and patients alike strive for optimal treatment outcomes. Prosthodontically driven implant placement has become the established and accepted method to obtain the most advantageous functional as well as aesthetic results. Prior to, or simultaneously with, implant placement it may be necessary to use bone augmentation procedures to rebuild or replace missing bone.  The alloplastic calcium phosphate based bone substitute materials are available in various formulations and methods of delivery. Hydroxyapatite is considered to show minimal resorption with time, however when the natural glycosaminoglycan, chondroitin sulphate, is incorporated into this material it has demonstrated increased bone regenerative capacity in vivo. Brushite bone cement is a more recent calcium phosphate material that appears to be superior for the purpose of bone grafting relative to hydroxyapatite because it allows new bone to be laid down more quickly.  To investigate the clinical potential of combining chondroitin-6-sulphate with brushite bone cements this material has been previously characterised. It was established that the incorporation of up to ten per cent chondroitin-6-sulphate into brushite does not affect its setting reaction or cause any chemical change in the set cement.  In the present study, it has been shown using scanning electron microscopy imaging to study cellular attachment, as well as the Alamar Blue® assay to study cellular proliferation, that cells can grow on brushite bone cements. The MG-63 osteosarcoma cell line was used for these in vitro experiments. The use of these osteoblast-like cells is an established model for investigating osteoblast function. Furthermore these experiments indicate that the concentration of chondroitin-6-sulphate present in the brushite cement does have an adverse effect upon the cellular response observed, in terms of both cellular attachment and proliferation.},
  keywords = {Alveolar bone grafting, implant dentistry, calcium phosphate bone cements, brushite.},
  note = Article ID: 730677
}