Abstract
Triglyceride (TG) is an important source of fuel during endurance exercise. Lipolysis of adipose tissue and intramuscular triacylglycerol oxidation increases during exercise. In compare with moderate-intensity exercise, high-intensity exercise decreases fat oxidation. For many years it has been debated whether triacylglycerols located in the muscle are utilized during exercise because conflicting results have been appeared. To conduct this research, 8 (eight) male mouse were taken as samples and four mouse were divided into two groups as control (sedentary life) and experimental (routine exercise in treadmill). After one month of close supervision, the mouse were seizured and collect the sample (liver, epidedymal adipose tissue, skeletal muscle) for further experiment. We analyzed the sample and estimate the TG (triglycerides) content from the sample with standard GPO-POD method. In this study, we found that compare with sedentary mouse, exercise group slightly increased liver weight and epididymal adipose tissue TG whereas gastrocnemius muscle weight increased significantly because of exercise induced muscle storage. On the other hand, in compare with body weight, epididymal adipose tissue weight and gastrocnemius muscle TG decreased significantly. There is no alteration in liver weight of both group. Finally, we demonstrate that exercise increased the skeletal muscle weight of trained mice and decreased the TG accumulation in gastrocnemius skeletal muscle and this findings is inversely correlated with insulin resistance.